Creating Cellular Micropatterns by Switching Fouling Properties of Electroactive Ito Surfaces
نویسندگان
چکیده
This paper describes a novel approach of forming micropatterned co-cultures using a combination of oxygen plasma ashing and electrochemical removal of poly(ethylene glycol) (PEG) silane from indium tin oxide (ITO) substrates. After assembling a layer of PEG silane on ITO, photolithography and oxygen plasma cleaning were used to form cell-adhesive domains within the non-fouling PEG silane layer. After patterning hepatocytes in these domains, the surrounding PEG silane layer was desorbed by applying negative voltage (-1.4 V vs. Ag/AgCl) to the underlying ITO substrate. This switched the fouling properties of the ITO substrate and allowed the adsorption of another cell type creating a micropatterned co-culture.
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